Journal: BMC Genetics
Article Title: DNA sequence polymorphisms within the bovine guanine nucleotide-binding protein Gs subunit alpha (Gsα)-encoding ( GNAS ) genomic imprinting domain are associated with performance traits
doi: 10.1186/1471-2156-12-4
Figure Lengend Snippet: Sequencing traces of NESP55 gene for genomic DNA and cDNA from both maternal and foetal tissues . The genomic DNA from the mother of each foetus allowed genotyping of the maternal genotype, while the genomic DNA from each foetus allowed genotyping of the each foetus. Arrows indicate the exonic SNP, denoted R (A/G), which was used for both DNA genotyping and to analyse allele-specific expression status of NESP55 transcripts (cDNA) across tissues from 8 and 10 weeks old foetuses. Column number 4 presents the genotypes for all samples/animals tested. Column 5 indicates whether monoallelic ( i.e . uniparental) expression was detected.
Article Snippet: All sequencing reactions were performed commercially by GATC Biotech Ltd. (Constance, Germany) using the primer sequences listed above and resulting DNA sequence traces were analysed using the LaserGene Package (DNASTAR, WI, USA).
Techniques: Sequencing, Expressing